1Cultivate Bacteria and Other Microbes in Liquid Culture and Solid Media
2Estimation of Lipids, Carbohydrates and Proteins (Bradford, Lowry's Method)
3Estimation of substrates alcohol, Acetic Acid, sugars
4High performance liquid chromatography (HPLC)
5Isolation of Secondary Metabolites and analysis by TLC
6Isolation of Carotenoids (and lipids) and Analysis by Thin Layer Chromatography (TLC)
7Assembly of bioreactors and Calibration of Probes (pH and Dissolved Oxygen)
8Sterilization
9Cascade control of Dissolved Oxygen
10Growth control
11Batch/Fed batch with concentrated feed and other metabolites by
12Continuous Stirred Tank Reactor running at different dilution rate
13Estimation of Growth/Product formation & Substrate utilization kinetics
14Estimation of Kla by dynamic gassing out and gas balancing.